吴迪  特聘研究员

职称职务： 特聘研究员、泰康生命医学中心PI

学科专业：生物化学与分子生物学

研究方向：多组学及蛋白质质谱学

实验室位置：医学部9号楼

Email： wudi1985@gmail.com

学习经历：

2010-2015 邓迪老员工命科学学院，博士

2007-2010 武汉老员工命科学学院，硕士

2003-2007 武汉老员工命科学学院，学士

主要工作经历与任职：

2024-今 mk体育 特聘研究员

2024-今 mk体育泰康生命医学中心 PI

2015-2024 牛津大学化学院，博士后

目前主要科学研究领域和兴趣：

· 基于结构质谱和多组学，发现并阐明癌症相关膜蛋白受体内源性配体及调控机理。

· 基于蛋白质质谱，研究癌症相关翻译后修饰对蛋白复合物的动态调控。

· 开发适用于检测单细胞膜蛋白复合物及其信号通路调控的高分辨率质谱新方法。

代表性论文（近5年，10篇以内的第一作者或通讯作者论文）

1. D. Wu, H. Tang, X. Qiu, S. Song, S. Chen, C. V. Robinson
Native MS guided lipidomics to define endogenous lipid micro-environments of eukaryotic receptors and transporters
Nat. Protoc. 2024 (Featured article)

2. D. Wu *, R. Yan, S. Song, A. K. Swansiger, Y. Li, J. S. Prell, Q. Zhou, C. V. Robinson * (Co-corresponding author)
The complete assembly of human LAT1-4F2hc complex provides insights into its regulation, function and localisation
Nat. Commun. 2024, 15, 3711 (Featured article in Editors’ Highlights)

3. Y. Yan, J. Xiao, F. Huang, W. Xian, B. Yu, R. Cheng, H. Wu, X. Lu, X. Wang, W. Huang, Jing Li, G. K. Oyejobi, C. V. Robinson, H. Wu, D. Wu*, X. Liu*, L. Wang*, B. Zhu* (Co-corresponding author)
Phage defence system CBASS is regulated by a prokaryotic E2 enzyme that imitates the ubiquitin pathway
Nat. Microbiol. 2024, 9, 1566-1578

4. D. Wu, M. Guo, C. V. Robinson
Connecting single nucleotide polymorphisms, glycosylation status and interactions of plasma serine protease inhibitors
Chem. 2023, 9, 665-681

5. D. Wu* and C. V. Robinson* (Co-corresponding author)
Native top-down mass spectrometry reveals a role for interfacial glycans on therapeutic cytokine and hormone assemblies
Angew. Chemie Int. Ed. 2022, 202213170, 1-8. (Cover)

6. D. Wu and C. V. Robinson
Understanding glycoprotein structural heterogeneity and interactions: insights from native mass spectrometry
Curr. Opin. Struct. Biol. 2022, 74, 102351.

7. D. Wu and C. V. Robinson
Connecting ‘multi-omics’ approaches to endogenous protein complexes
Trends in Chem. 2021, 3, 445-455. (Cover)

8. D. Wu, J. Li, W. B. Struwe and C. V. Robinson
Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis
Chem. Sci. 2019, 10, 5146-5155.

9. D. Wu, W. B. Struwe, D. J. Harvey, M. A. J. Ferguson and C. V. Robinson
N-glycan microheterogeneity regulates interactions of plasma proteins
Proc. Natl. Acad. Sci. 2018, 115, 8763–8768.

10. D. Wu*, W. Struwe* (Co-corresponding author)
Native mass spectrometry of glycoproteins
New Developments in Mass Spectrometry: Glycoprotein Analysis 2024 (Book chapter)

重要合作论文

1. P. A. M. Schmidpeter, D. Wu, J. Rheinberger, P. M. Riegelhaupt, H. Tang, C. V. Robinson and C. M. Nimigean
Anionic lipids unlock the gates of select ion channels in the pacemaker family
Nat. Struct. Mol. Biol. 2022, 121, 438a-439a.

2. S. Chen, T. Getter, D. Salom, D. Wu, D. Quetschlich, D. Chorev, K. Palczewski, C. V. Robinson
Capturing a G-protein coupled receptor signaling cascade across a native membrane
Nature 2022, 604, 384–390.

3. D. N. Patil, S. Singh, T. Laboute, T. Strutzenberg, X. Qiu, D. Wu, S. J. Novick, E. S. Rangarajan, C. V. Robinson, P. R. Griffin, J. F. Hunt, T. Izard, A. K. Singh, K. A. Martemyanov
Cryo-EM structure of human GPR158 receptor coupled to the RGS7-Gβ5 signaling complex
Science 2022, 375, 86–91

4. L. Wang, D. Wu, C. V. Robinson, H. Wu, T. Fu
Structures of a complete human V-ATPase reveal mechanisms of its assembly
Mol. Cell 2020, 80, 501-511.e3.

5. D. S. Chorev, H. Tang, S. L. Rouse, J. R. Bolla, A. Kügelgen, L. A. Baker, D. Wu, J. Gault, K. Grünewald, T. A.M. Bharat, S. J. Matthews and C. V. Robinson.
The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes
Nat. Protoc. 2020, 15, 1690-1706.

6. Y. M. Abbas, D. Wu, S. A. Bueler, C. V Robinson and J. L. Rubinstein
Structure of V-ATPase from the mammalian brain
Science 2020, 367, 1240-1246.

7. A. D. Malay, N. Miyazaki, A. Biela, S. Chakraborti, K. Majsterkiewicz, I. Stupka, C. S. Kaplan, A. Kowalczyk, B. M. A. G. Piette, G. K. A. Hochberg, D. Wu, T. P. Wrobel, A. Fineberg, M. S. Kushwah, M. Kelemen, P. Vavpetič, P. Pelicon, P. Kukura, J. L. P. Benesch, K. Iwasaki, J. G. Heddle
An ultra-stable gold-coordinated protein cage displaying reversible assembly and paradoxical geometry
Nature 2019, 569, 438-442.

8. D. S. Chorev, L. A. Baker, D. Wu, V. Beilsten-Edmands, S. L. Rouse, T. Zeev-Ben-Mordehai, C. Jiko, F. Samsudin, C. Gerle, S. Khalid, A. G. Stewart, S. J. Matthews, K. Grünewald, and C. V. Robinson
Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry
Science 2018, 362, 829–834.

9. H-Y Yen, K. K. Hoi, I. Liko, G. Hedger, M. R. Horrell, W. Song, D. Wu, P. Heine, T. Warne, Y. Lee, B. Carpenter, A. Plückthun, C. G. Tate, M. S. P. Sansom and C. V. Robinson
PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling
Nature 2018, 559, 423-427.

10. J. R. Bolla, J. B. Sauer, D. Wu, S. Mehmood, T. M. Allison and C. V. Robinson
Direct observation of the influence of cardiolipin and antibiotics on lipid II binding to MurJ
Nat. Chem. 2018 Mar; 10(3): 363–371.